MockV® RVLP Kit (M230)

Item Number Unit Size Qty
M230 1 kit


Winner of the 2023 R&D 100 Award

The Cygnus MockV® RVLP Kit is based on a BSL-1 compatible stock solution of non-infectious Retrovirus-like Particles (RVLP), derived endogenously from CHO cell culture, as a spiking agent for viral clearance testing. From your own bench, predict retroviral validation outcomes, incorporate viral clearance into process development/ optimization experiments and employ viral clearance to QbD, DOE and HTS approaches. Instead of relying solely on a CRO-provided model XMuLV retrovirus, you can now independently spike and assess the removal of the original retrovirus-like particle of regulatory concern1

The MockV® RVLP Kit includes a vial of RVLP stock solution, a 96-well plate for sample analysis, RNA extraction and qPCR reagents, and a well-controlled RNA standard for accurate and reliable RVLP quantification. By following the kit’s easy-to-use protocol, scientists can detect as little as 1 x 103 RVLP/mL, enabling an LRV of ~ 5.0 to be determined.  Each kit contains 2.0 mL of RVLP Stock Solution at a concentration of 1 x 1010 RVLP/mL, sufficient for spiking up to 200 mL of load material to 1% (v/v), and quantification components for conducting analysis on 23 samples in triplicate. In less than one day, the assay and data analysis can be performed.  A real-time qPCR instrument is required along with standard laboratory equipment.  Minimal experience with RNA extraction or qPCR protocols is required. Compatible purification steps include Protein A chromatography, virus filtration, anion and cation exchange chromatography, mixed-mode, hydrophic-interaction and size- exclusion chromatography. The MockV® RVLP Kit achieves LRV accuracy to within ± 0.5 for these modes of separation as compared to traditional viral clearance studies with XMuLV model virus.

Now, through the use of Cygnus’s BSL-1 compatible viral clearance kits, you can easily and economically quantify viral clearance for downstream process steps in your own lab, on your timeline.



QbD = Quality by Design

DOE = Design of Experiment

HTS = High-throughput Screening



1. Anderson, Kevin P., et al. "Endogenous origin of defective retrovirus-like particles from a recombinant Chinese hamster ovary cell line." Virology1 (1991): 305-311.

Product Information

Product Type:
Viral Clearance Kit
RVLP sRNA at -80°C, Endonuclease and qPCR Reagents at -20°C, RVLP Stock Solution at 4°C, RNA Extraction Reagents at 4°C, and Deep Well Extraction Plate at room temperature
Assay format:
96 Well Plate

Kit Components

Endonuclease Buffer
RVLP Processing Buffer
Proteinase K
RNA Extraction Buffer
RNA Precipitation Buffer
RNA Wash Buffer
RNA Reconstitution Buffer
Deep Well Extraction Plate with Mat
RVLP Master Mix
RVLP Forward Primer
RVLP Reverse Primer
RVLP Probe
PCR Grade Water
RVLP sRNA (lyophilized)

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