Use the specific antigen concentrates as controls in ELISA testing, for spike and recovery experiments, or in western blot analysis.
Most of the HCP antigen concentrates are extracted from crude sources, such as lysate or culture media, and may have been further processed or purified. Absolute quantification of these complex antigens mixtures is difficult. Concentrations and fill volumes may vary according to current lot availability.
The potency and stability of these antigens in various dilution matrices has not been established. For these reasons, an approximate concentration is listed on the label. It is the responsibility of the end user to determine the final concentration of these antigens under their conditions of use and relative to these standards/calibrators available for the assay.