IsoTag™ AAV — Residual Reagent ELISA (F1035)

Item Number Unit Size Price Qty
F1035 1 kit


The IsoTag™ AAV Residual Reagent assay is a two-site immuno-enzymatic assay intended to quantify IsoTag™ AAV Residual Reagent. The F1035 kits incorporate a well-qualified sample treatment method to dissociate the IsoTag™ AAV Residual Reagent from the AAV virus. Samples potentially containing residual IsoTag™ AAV Reagent are mixed with sample denaturing buffer to dissociate the biopolymer from the AAV virus. The samples are then reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-IsoTag™ AAV Reagent antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture antibody (goat polyclonal). After a wash step to remove any unbound reagents, the strips are then reacted with tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of IsoTag™ AAV Residual Reagent present in the sample. This immunoassay method provides sensitivity to the IsoTag™ AAV Residual Reagent to less than 1ng/mL. This kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process manufacturing as well as final product.

Learn more about Isolere Bio IsoTag™ Technology here.

Product Information

Product Type:
2°C to 8°C
Assay format:
96 Well Plate
Time to result:
~1 hr. 45 min
~0.08 ng/mL
~0.16 ng/mL
Recommended Diluent:

Kit Components

Anti-IsoTag™ AAV Reagent:HRP
IsoTag™ AAV Reagent Standards
Anti-IsoTag™ AAV Reagent coated microtiter strips

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?
  • Calibration of HCP assays