HEK 293 HCP ELISA Kit, 3G (F650S)

Item Number Unit Size Price Qty
F650S 1 kit


Expression of viral vectors in HEK 293 cells is a widely used procedure for obtaining sufficient quantities of a desired virus. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins (HCPs) from HEK 293 cells. HCP impurities can result in adverse toxic or immunological reactions and thus it is desirable to reduce these impurities to the lowest levels practical.

This HEK 293 HCP ELISA Kit, 3G (F650S) can be used as a process development tool to monitor the optimal removal of host cell impurities as well as in routine final product release testing when comprehensively characterized using samples from your process. The polyclonal antibodies used in this kit have been generated against and affinity-purified using a mild lysate of HEK 293 cells to obtain HCPs typically encountered in initial product recovery steps. The antibodies have broad coverage of ~90% to HEK 293 HCPs as determined by Antibody Affinity Extraction with 2D PAGE/Silver Stain and LC-MS. The kit provides the specificity and sensitivity (LOD ~0.35 ng/ml, LLOQ ~2 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance. Please use Sample Diluent, Cat. No. I700, with these kits.

Please note that the original pool of anti-HEK 293 HCP antibody has been depleted by Dec 31, 2021. The new Resupply 1 antibodies for an ELISA format to match the original F650S kit performance as close as possible were raised and the resupply HEK 293 HCP ELISA Kit, 3G replaced the original HEK 293 HCP ELISA Kit, 3G. Kits containing the new resupply antibodies maintains the same catalog number, F650S, and is identified by the lot numbers starting with characters “R1”.

Both Cygnus F650S HEK 293 HCP ELISA Kits, 3G, Original and Resupply 1, have gained global regulatory approvals for multiple commercially available gene and cell therapies.

Important Change Notification Memo: HEK 293 HCP ELISA Kit, 3G Resupply 1

Product Information

Product Type:
Standards -10°C to -30°C upon receipt, All other kit reagents 2°C to 8°C
Target Expression System:
HEK 293
Species Group:
Assay format:
96 Well Plate
Time to result:
~2 hrs. 20 min
~0.35 ng/mL
~2.0 ng/mL
Recommended Diluent:

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?
  • Calibration of HCP assays