HEK 293 HCP ELISA Kit, 3G (F650S)

The Original F650S Kits is no longer available due to its capture and detection antibody stock depletion. If ordering from this page, you will receive the F650S Resupply 1. Please contact [email protected] should you have any questions or require further assistance.

Item Number Unit Size Price Qty
F650S 1 kit


Important Change Notification Memo: HEK 293 HCP ELISA Kit, 3G Resupply 1

Expression of viral vectors in HEK 293 cells is a widely used procedure for obtaining sufficient quantities of a desired virus. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins (HCPs) from HEK 293 cells. HCP impurities can result in adverse toxic or immunological reactions and thus it is desirable to reduce these impurities to the lowest levels practical.

The polyclonal antibodies used in this kit have been generated against and affinity-purified using a mild lysate of HEK 293 cells to obtain HCPs typically encountered in initial product recovery steps. This kit can be used as a process development tool to monitor the optimal removal of host cell impurities as well as in routine final product release testing when comprehensively characterized using samples from your process.

The new Resupply 1 antibodies have recently been generated for an ELISA format to match the current F650S kit performance as close as possible. Similar to the anti-HEK 293 HCP antibodies used in the current F650S kit, the new antibodies have broad coverage of ~90% to HEK 293 HCPs as determined by Antibody Affinity Extraction with 2D PAGE/Silver Stain and LC-MS. The HEK 293 HCP ELISA Kit, 3G, Resupply 1, Cat. No. F650S, will replace the current HEK 293 HCP ELISA Kit, 3G, Cat. No F650S. Kits containing the new resupply antibodies will maintain the same catalog number, F650S, and will be identified by the lot numbers starting with characters “R1”. Both kits follow the same assay protocol and have LOQ of 1 ng/mL. Please use Sample Diluent, Cat. No. I700, with these kits.

Product Information

Product Type:
Standards -10°C to -30°C upon receipt, All other kit reagents 2°C to 8°C
Target Expression System:
HEK 293
Species Group:
Assay format:
96 Well Plate
Time to result:
~2 hrs. 20 min
~0.35 ng/mL
~2.0 ng/mL
Recommended Diluent:

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • Calibration of HCP assays
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?

Related Products