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SP2/0 HCP ELISA Kit, 2G - (F1030)

Product insert

Item Number Unit Size Price Qty
F1030 1 kit
$924.00
Item Number Unit Size Price Qty
I028-1000 1000 ml
$776.00
I028-500 500 ml
$461.00
I028-100 100 ml
$174.00

Product insert

Description

The SP2/0 HCP ELISA Kit, 2G is a simple-to-use, objective, and semi-quantitative ELISA kit  intended for use in determining the presence of host cell protein impurities in products manufactured by expression in SP2/0 cells. 

The antibodies used in this 2nd generation SP2/0 HCP ELISA Kit, F1030, have been raised against and affinity purified using HCP recovered from culture of SP2/0 null cells in a protein-additive-free media. The antibodies were characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, demonstrating 83% coverage to HCPs in SP2/0 null culture. This kit has LOD ~0.4 ng/ml and LLOQ ~3ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. 

This kit will be replacing Cygnus SP2/0 HCP ELISA Kit, Item # F180. 

Product details
Product Type ELISA Kit
Recommended Storage 2°C to 8°C
Target Expression System SP2/0
Species Group Mammalian
Species Mouse
Assay format 96 Well Plate
Time to result ~2 hrs. 50 min
LOD ~0.4 ng/mL
LLOQ ~3 ng/mL
Recommended Diluent I028
Kit Components
Anti-SP2/0 Coated Microtiter Strips, 2G
Anti-SP2/0:HRP Conjugate, 2G