PER.C6® HCP ELISA Kit, 2G (F1055)

Item Number Unit Size Price Qty
F1055 1 kit


The PER.C6® HCP ELISA Kit, 2G is a simple-to-use, objective, and semi-quantitative ELISA kit  intended for use in determining the presence of host cell protein impurities in products manufactured by expression in PER.C6 cells. 
The antibodies used in this 2nd generation PER.C6 HCP ELISA Kit, F1055, have been raised against and affinity purified using using PER.C6 cell line HCPs found in protein free conditioned media. The antibodies were characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, demonstrating reactivity to majority of HCPs found in PER.C6 expression system processes. This kit has LOD ~1.2 ng/ml and LLOQ ~5ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. 

This kit will be replacing Cygnus PER.C6 HCP ELISA Kit Item # F530. 

PER.C6 cell line is a registered trademark of JANSSEN VACCINES & PREVENTION B.V.

Product Information

Product Type:
Standards -10°C to -30°C upon receipt, All other kit reagents 2°C to 8°C
Target Expression System:
Species Group:
Assay format:
96 Well Plate
Time to result:
~2 hrs. 50 min
~1.2 ng/mL
~5 ng/mL
Recommended Diluent:

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?
  • Calibration of HCP assays