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E. coli HCP ELISA Kit, 2G - (F1020)

Product insert

Item Number Unit Size Price Qty
F1020 1 kit
$924.00
Item Number Unit Size Price Qty
I028-1000 1000 ml
$776.00
I028-500 500 ml
$461.00
I028-100 100 ml
$174.00

Product insert

Description

This E. coli HCP 2nd Generation ELISA Kit, Item Number F1020, is intended to replace the current E. coli HCP ELISA Kit, Item Number F410. Cygnus has manufactured, characterized, and qualified large pools of the new reagents that will replace the current reagents. The antibodies were generated against and affinity purified using mild lysate from a number of widely used E. coli strains, similarly to the original critical reagents in the F410 Kit. The coverage of the new antibodies to the E. coli HCP antigen was determined to be ~87-88% by Antibody Affinity Extraction with Mass Spectrometry (AAE-MS™). More importantly, Cygnus Technologies has demonstrated the equivalency of the new antibodies (F1022-AF) in the E. coli HCP ELISA, 2G (F1020) using a panel of E. coli samples from several independent biomanufacturing processes. For BL21(DE3) processes, a BL21(DE3) E. coli ELISA Kit is also now available (F1060).  

This E. coli HCP ELISA Kit, 2G provides the specificity and sensitivity (LOD ~0.3 ng/ml, LLOQ ~3 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance, aiding in optimal purification process development, process control, routine quality control, and product release testing. Please use Sample Diluent, Item No. I028, with these kits; available separately.

Important Change Notification Memo: E. coli HCP ELISA Kit, 2G 

Updated F410 Memo, 30 Sep 2024: Continued Supply of the Cygnus Technologies E. coli HCP ELISA, Cat# F410

New BL21(DE3) HCP ELISA Kit, Item # F1060, Now Available 

Product details
Product Type ELISA Kit
Recommended Storage 2°C to 8°C
Target Expression System E. coli
Species Group Bacterial
Species E. coli
Assay format 96 Well Plate
Time to result ~2 hrs. 20 min
LOD 0.3 ng/mL
LLOQ ~ 3 ng/mL
Recommended Diluent I028