BHK HCP ELISA Kit (F510)

Item Number Unit Size Price Qty
F510 1 kit
$872.00

Description

The BHK (baby hamster kidney fibroblast) cell line is used as an expression system for viral vaccines and other therapeutic proteins. Our HCP kit is generic in the sense that it is intended to react with essentially all of the HCPs that could contaminate the product, independent of purification process. The antibodies were generated against and affinity purified using a mild lysate of BHK cells. The resulting antibodies were then been characterized against three commercial cell lines used to produce various viral and protein products. This analysis indicated the vast majority of HCPs were conserved among multiple BHK cell lines and product purification processes. Our assay qualification determined the LOD for this kit is ~0.4 ng/ml. Please use Sample Diluent, Item No. I028, with these kits; available separately.

Product Information

Product Type:
ELISA Kit
Storage:
Standards -10°C to -30°C upon receipt, All other kit reagents 2°C to 8°C
Target Expression System:
BHK
Species Group:
Mammalian
Species:
Syrian golden hamster
Assay format:
96 Well Plate
Time to result:
~2 hrs. 50 min
LOD:
0.4 ng/mL
LLOQ:
1 ng/mL
Recommended Diluent:
I028

Product Insert

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?
  • Calibration of HCP assays