Western blot detection kits are used to demonstrate the reaction of HCP antibodies with the majority of HCPs present in a given cell line or purification process. Due to sensitivity limitations, however, coverage can only be estimated on upstream, harvest samples where HCPs are still within the sensitivity limitations of various staining methods.
Western blot detection kits provide limited predictive value in determining how the ELISA using that antibody will quantitatively react to the more limited array of HCPs in downstream samples.
Please refer to our Custom Development Services pages to learn about Antibody Affinity Extraction (AAE) - a superior method for antibody coverage analysis and identification of individual HCPs that might co-purify with the drug substance.
Western Blot Kits for identifying Host Cell Proteins from a number of mammalian cell lines including CHO, NS/0, SP2/0 and A549 cells. The antibodies used in these kits are the same as those used in our corresponding ELISA kits.
Western Blot Kits for identifying P. pastoris and S.cerevisiae Host Cell Proteins. The antibodies used in these kits are the same as those used in our corresponding ELISA kits.
Western Blot Kit for identification of total goat milk proteins. The antibodies used in this kit are the same as those used in our corresponding ELISA kit.
Western Blot Kit for identifying E. coli Host Cell Proteins. The antibodies used in this kit are the same as those used in our corresponding ELISA kit.
