Protein A ELISA Kit (F400)

Item Number Unit Size Price Qty
F400 1 kit


This ELISA detects unnatural constructs of Protein A, such as the MabSelect SuRe™ ligand sold by GE Healthcare. Use this kit if you are using a recombinant form of Protein A with significant structural differences from natural Protein A such as MabSelect SuRe™.

Four newer Protein A assays known as Mix-N-Go™ methods are also available. These methods replace the sample boiling and centrifugation steps with a special sample treatment reagent that allows accurate detection of Protein A in the presence of your sample antibody. The Protein A Mix-N-Go™ kit (F610) uses standards calibrated to unnatural constructs of Protein A such as GE Healthcare's MabSelect SuRe™. The Protein A Mix-N-Go™ kit (F600) uses standards calibrated against natural and conserved recombinant forms of Protein A. 

The LOD for this F400 kit is ~50 pg/ml. Please use Sample Diluent, Item No. I028, with these kits; available separately.

Product Information

Product Type:
All kit reagents 2°C to 8°C
Assay format:
96 Well Plate
Time to result:
~3 hrs. 40 min
~50 pg/mL
100 pg/mL
Recommended Diluent:

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Which Cygnus Protein A ELISA kit to use: F050H, F400, F400Z, F600 or F610?
  • Why Perform Dual Wavelength Analysis?