The NS/0 HCP ELISA Kit, 2G is a simple-to-use, objective, and semi-quantitative ELISA kit  intended for use in determining the presence of host cell protein impurities in products manufactured by expression in NS/0 cells. 

The antibodies used in this 2nd generation NS/0 HCP ELISA Kit, F1085, have been raised against and affinity purified using HCP recovered from culture of NS/0 null cells in a protein-additive-free media. The antibodies were characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, demonstrating 73% coverage to HCPs in NS/0  null culture. This kit has LOD ~0.75 ng/ml and LLOQ ~3ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. Please use Sample Diluent, Item No. I028, with these kits; available separately.

This kit will be replacing Cygnus NS/0 HCP ELISA Kit, Item # F220.