This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression in E. coli. 
The D1415 kit includes all the necessary reagents and consumables for quantitative PCR from extracted DNA samples obtained by your extraction method of choice. Cygnus offers DNA Extraction Kits (D100T and D100W) developed specifically for efficient and reproducible host cell DNA extraction. The qPCR reagents included in the D1415 kit feature a state-of-the-art master mix containing CleanAmp^® dNTPs and Hot Start Taq DNA Polymerase to prevent non-specific priming during PCR setup, and an E. coli-specific primer/probe mix containing FAM-labeled probes that are quenched with BHQ-1™ until PCR extension. The kit includes E. coli DNA concentrate and TE buffer for setting up standard curves and diluting your sample if necessary. This kit is compatible with a variety of qPCR instruments, enabling analysis using your software of choice.

Licensing Information

CleanAmp^® is a registered trademark of TriLink BioTechnologies LLC. CleanAmp^® dNTPs contained herein are provided with a non-transferable right from TriLink BioTechnologies, LLC for use in AccuRes™ DNA Quantification Kits and AccuRes™ Quantitative DNA kits.

'Black Hole Quencher' and 'BHQ-1' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.