Vero Cell 2G HCP ELISA Kit (F975)

Item Number Unit Size Price Qty
F975 1 kit


Expression of viral vectors and vaccines in Vero cells is a cost-effective method for production of commercial quantities of a drug substance. The manufacturing and purification process of these products leaves the potential for impurities by HCPs from Vero cells. Such impurities can reduce the efficacy of the therapeutic or vaccine agent and result in adverse toxic or immunological reactions. Thus, it is desirable to reduce HCP impurities to the lowest levels practical.
This kit was developed using the antibodies that have been generated against and affinity purified using mild lysate of Vero cells. This simple to use, robust, and semi-quantitative ELISA is a sensitive and specific method to aid in optimal purification process development, process control, routine quality control, and product release testing. This kit is “generic” in the sense that it is intended to react with a majority of the HCPs that could pollute the product independent of the purification process.
This Vero Cell HCP ELISA has an LOD of ~1.4 ng/ml. Please use Sample Diluent, Item No. I028, with these kits; available separately.

Product Information

Product Type:
2°C to 8°C
Target Expression System:
Vero Cell
Species Group:
African green monkey
Assay format:
96 Well Plate
Time to result:
~2 hrs. 50 min
~1.4 ng/mL
~ 3 ng/mL
Recommended Diluent:

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • Calibration of HCP assays
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?