The Sf9 HCP ELISA Kit, 3G is a simple-to-use, objective, and semi-quantitative ELISA kit intended for use in determining the presence of host cell protein impurities in products manufactured by expression in Sf9 insect cells.
The antibodies used in this 3nd generation Sf9 HCP ELISA Kit, F1040, have been raised against and affinity purified using a mild lysate of Sf9 cells to obtain HCPs typically encountered in your initial product recovery step. The antibodies were characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, demonstrating reactivity to majority of HCPs in Sf9 cell lysate. This kit has LOD ~0.3 ng/ml and LLOQ ~3ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs.
This kit will be replacing Cygnus Sf9 HCP ELISA Kit, 2G, Item # F840.