Sf9 HCP ELISA Kit, 3G (F1040)

Item Number Unit Size Price Qty
F1040 1 kit


The Sf9 HCP ELISA Kit, 3G is a simple-to-use, objective, and semi-quantitative ELISA kit  intended for use in determining the presence of host cell protein impurities in products manufactured by expression in Sf9 insect cells. 
The antibodies used in this 3nd generation Sf9 HCP ELISA Kit, F1040, have been raised against and affinity purified using a mild lysate of Sf9 cells to obtain HCPs typically encountered in your initial product recovery step. The antibodies were characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, demonstrating reactivity to majority of HCPs in Sf9 cell lysate. This kit has LOD ~0.3 ng/ml and LLOQ ~3ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. 

This kit will be replacing Cygnus Sf9 HCP ELISA Kit, 2G, Item # F840. 

Product Information

Product Type:
2°C to 8°C
Target Expression System:
Species Group:
S. frugiperda
Assay format:
96 Well Plate
Time to result:
~2 hrs. 20 min
0.3 ng/mL
~ 3 ng/mL
Recommended Diluent:

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?
  • Calibration of HCP assays