Troubleshooting & FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • Custom Assay & Antibody Development Services
  • Calibration of HCP assays
  • How do I Maintain Quality Control of ELISA?
  • How do I Perform Protein A Sample Treatment Procedure?
  • How do I Perform Spike & Recovery Studies?
  • How do I Qualify HCP & Bioprocess Impurity Assays?
  • What About Automation of Immunoassays?
  • What About Cross Reactivity of anti-HCP Antibodies to Drug Substances?
  • What are the Allowable Limits of HCP Impurities?
  • What are the best curve fitting routines for ELISA?
  • What Custom Services does Cygnus Provide?
  • What is "High Dose Hook Effect"?
  • What is Matrix Interference?
  • What is optimal, Process-Specific versus Generic/Platform/Multi-Use HCP Assays?
  • What is the difference in Sensitivity and Specificity of Western Blot vs ELISA?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Which Cygnus CHO HCP kit to use: F015, CM015, F550 or F550-1?
  • Which Cygnus Insulin ELISA Kit to use, F040 or F280?
  • Which Cygnus Protein A ELISA kit to use: F050, F050H, F400, F400Z, F600 or F610?
  • Which Sample Diluents should be used?
  • Why is Dilution Linearity important?
  • Why Perform Dual Wavelength Analysis?
  • How does MVP LRV data compare to live MVM spiking studies?
  • How can you ensure that the Spiking MVP is BSL-1 compatible?
  • Can this technique be used for predicting viral inactivation?
  • How many experiments can I perform with the MockV MVM Kit?
  • How much MVP should I challenge my load with?
  • What is the Immuno-qPCR assay’s LOQ?
  • What is the shelf life of the MVP?
  • How can I ensure that my sample matrices will not interfere with the Immuno-qPCR assay?
  • When analyzing for assay interference, what spike/recovery percentages are acceptable?
  • How should I store my experimental samples prior to Immuno-qPCR analysis?
  • How should I clean my column after an MVP spiking experiment?