Troubleshooting & FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • Custom Assay & Antibody Development Services
  • Calibration of HCP assays
  • How do I Maintain Quality Control of ELISA?
  • How do I Perform Protein A Sample Treatment Procedure?
  • How do I Perform Spike & Recovery Studies?
  • How do I Qualify HCP & Bioprocess Impurity Assays?
  • What About Automation of Immunoassays?
  • What About Cross Reactivity of anti-HCP Antibodies to Drug Substances?
  • What are the Allowable Limits of HCP Impurities?
  • What are the best curve fitting routines for ELISA?
  • What Custom Services does Cygnus Provide?
  • What is "High Dose Hook Effect"?
  • What is Matrix Interference?
  • What is optimal, Process-Specific versus Generic/Platform/Multi-Use HCP Assays?
  • What is the difference in Sensitivity and Specificity of Western Blot vs ELISA?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Which Cygnus CHO HCP kit to use: F015, CM015 or F550?
  • Which Cygnus Insulin ELISA Kit to use, F040 or F280?
  • Which Cygnus Protein A ELISA kit to use: F050, F050H, F400, F400Z, F600 or F610?
  • Which Sample Diluents should be used?
  • Why is Dilution Linearity important?
  • Why Perform Dual Wavelength Analysis?