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Protein A ELISA kit

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Protein A ELISA kit

This ELISA is designed to overcome low analytical recovery of Protein A encountered in many commercial kits. Most low recovery samples are either incomplete antibody fragments or so called fusion proteins where the antibody structure is linked to another non-antibody molecule. The properties of such unnatural drug substance constructs means that conventional sample treatment procedures are ineffective in dissociation, denaturation, precipitation and removal of the drug substances prior to using the Protein A detection assay. Any un-denatured drug substance remaining in the sample after the treatments step can re-associate with leached Protein A, resulting in under recovery by ELISA.

The #F400Z Protein A ELISA kit has been qualified using several problematic samples and found to yield acceptable recovery on all. This kit uses the same antibodies standards and sample treatment protocol as in the #F400 except that this kit contains a "Recovery Enhancer" solution which is combined with the HRP conjugated anti-Protein A antibody, #F410Z prior to use. The "Recovery Enhancer" prevents the re-association of any drug substance that was not completely removed during the sample treatment step. The #F400Z will accurately quantitate the unnatural construct of Protein A such as the MabSelect SuReTM as well as other conserved, natural and recombinant Protein A constructs. Because not all drug substances will respond equally to sample treatment procedures, each new sample type and drug substance must be validated for acceptable analytical recovery before reporting results.

Since this kit has been released 2 newer Protein A assays, known as Mix-N-Go, methods have been developed. These new methods eliminate the sample boiling and centrifugation steps. Instead, a special sample treatment reagent is used that allows for accurate detection of Protein A in the presence of your sample antibody. The Cat. #F600 kit uses standards calibrated against natural and conserved recombinant forms of Protein A. The Cat. #F610 kit uses standards calibrated to unnatural constructs of Protein A such as GE Healthcare's MabSelect SuReTM.

Catalog # Size Price Qty.
F400Z 1 kit $625.00