CHO Lysate Host Cell Protein ELISA Kit , 2G (F1045)

Item Number Unit Size Price Qty
F1045 1 kit
$1,001.00

Description

The CHO Lysate HCP ELISA Kit, 2G is a simple-to-use, objective, and semi-quantitative ELISA kit  intended for use in determining the presence of host cell protein impurities in products manufactured by expression in CHO cells. This kit is specifically designed to monitor HCP in a process where the CHO cells are lysed as part of the downstream process to recover the product protein of interest. Cygnus CHO HCP ELISA, 3G, Item # F550-1, should be used when the protein of interest is secreted into the culture media. 

The antibodies used in this 2nd generation CHO Lysate HCP ELISA Kit, F1045, have been raised against and affinity purified using a mild lysate of CHO cells. The antibodies were characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, demonstrating 96% coverage to HCPs in CHO lysate. This kit has LOD ~0.7 ng/ml and LLOQ ~3ng/ml. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. 

This kit will be replacing Cygnus CHO HCP ELISA Kit, Item # F015. 

Product Information

Product Type:
ELISA Kit
Storage:
2°C to 8°C
Target Expression System:
CHO
Species Group:
Mammalian
Species:
Chinese hamster
Assay format:
96 Well Plate
Time to result:
~3 hrs. 50 min
LOD:
~0.7 ng/mL
LLOQ:
~3 ng/mL
Recommended Diluent:
I028

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • Calibration of HCP assays
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?