This kit is designed for sensitive and specific quantification of residual host cell DNA present in upstream/downstream process samples or drug products manufactured by recombinant expression in CHO cell lines.
The kit includes reagents to extract, precipitate and resuspend residual host cell DNA from up to 96 samples (in a deep well plate) for downstream qPCR analysis, as well as all the necessary reagents and consumables for quantitative PCR. The qPCR reagents include a state-of-the-art master mix containing CleanAmp® dNTPs and Hot Start Taq DNA Polymerase to prevent non-specific priming during PCR setup, and a CHO cell line-specific primer/probe mix containing FAM-labeled probes that are quenched with BHQ-1™ until PCR extension. CHO DNA concentrate is included for standard curve generation. This This standalone kit is compatible with a variety of qPCR instruments, enabling analysis using your software of choice.
Licensing Information
CleanAmp® is a registered trademark of TriLink BioTechnologies LLC. CleanAmp® dNTPs contained herein are provided with a non-transferable right from TriLink BioTechnologies, LLC for use in AccuRes™ DNA Quantification Kits.
'Black Hole Quencher' and 'BHQ-1' are trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ dye technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc. Products incorporating the BHQ dye moiety are sold exclusively for R&D use by the end-user. They may not be used for clinical or diagnostic purposes and they may not be re-sold, distributed or re-packaged.
