Avitide AVIPure® — AAV8 Residual Ligand Assay (F1005)

Item Number Unit Size Price Qty
F1005 1 kit


The Avitide AVIPure® Residual Ligand Assay kits are intended for use in quantitating the Avitide AVIPure®-AAV8 Ligand. This kit (Item Number F1005) incorporates a well-qualified sample treatment method to dissociate the ligand from the AAV virus.
AVIPure-AAV8 Ligand, immobilized on various chromatography media, is used to purify AAV8 virus. Even when covalently attached, the ligand can leach off of the chromatography support and co-elute with AAV8. Residual AVIPure-AAV8 Ligand must be minimized to avoid any adverse patient effects.
The Avitide AVIPure® — AAV8 Ligand assay is a two-site immuno-enzymatic assay. Samples containing AVIPure® — AAV8 Ligand are mixed with sample denaturing buffer to dissociate the ligand from the AAV virus. The samples are then reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-AVIPure® antibody in microtiter strips coated with an affinity purified capture antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-Ligand-enzyme labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of AVIPure® — AAV8 Ligand present in the sample. Accurate quantitation is achieved by comparing the signal of unknowns to AVIPure® — AAV8 Ligand standards assayed at the same time.
This immunoassay provides a simple to use, precise, and highly sensitive method to detect AVIPure-AAV8 Ligand to less than 1ng/mL. As such, it can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

Please contact Repligen to inquire about AVIPure® – AAV8 Resin.

Product Information

Product Type:
2°C to 8°C
Assay format:
96 Well Plate
Time to result:
~2 hrs. 50 min
~0.06 ng/mL
~0.5 ng/mL
Recommended Diluent:

Kit Components

Sample Denaturing Buffer
Anti-AVIPure® — AAV8 Ligand: HRP
Anti-AVIPure® coated microtiter
AVIPure® — AAV8 Ligand Standards

Product FAQs

  • Can the ELISA Protocol in the Product Insert be Modified?
  • Calibration of HCP assays
  • How do I Maintain Quality Control of ELISA?
  • What About Automation of Immunoassays?
  • What is the Storage and Stability of ELISA Kits?
  • What Issues to Consider When Diluting Samples?
  • Why Perform Dual Wavelength Analysis?

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