These kits are important analytical tools used for the detection and identification of HCPs from commonly used recombinant expression systems.  The antibodies in the Western Blot kits are the same ones used in our corresponding ELISA kits.  Western Blot assays are helpful in demonstrating that the antibodies react with the majority of HCPs present in a given cell line or purification process.  Because of the limited sensitivity with Western Blot detection methods, they are usually only able to qualitatively detect HCPs from samples upstream in the purification process.  For more sensitive and semi-quantitative detection of HCPs it is strongly recommended that the corresponding ELISA kit be employed.  In general, ELISA kits have approximately 100 fold more sensitivity, and as such should be capable of detecting less than part per million total HCP levels in downstream and final product.

These complete Western Blot kits are sufficient for at least 5 gels and contain:

- Direct enzyme conjugated antibodies

 - Substrate

 - Positive control antigen

 - Block wash concentrate

Click on the product of interest to view the technical insert. 

Product

Catalog Number

A549 HCP Western Blot Kit

F235

CHO HCP Western Blot kit (Lysate)

F060

CHO-CM HCP Western Blot kit

CM060

E.coli HCP Western Blot kit (HRP)

F415

Goat Milk Proteins Western Blot kit

F245

HEK 293 HCP Western Blot kit

F155

Lactococcus lactic HCP Western Blot kit

F495

NS/0 HCP Western Blot kit

F225

Pichia pastoris HCP Western Blot kit

F145

Saccharomyces cerevisiae HCP Western Blot kit

F130

SF9  HCP Western Blot kit

      F125      

SP2/0 HCP Western Blot kit

F185