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This ELISA incorporates some improvements over the earlier kits and is designed to better detect the newer, unnatural constructs of Protein A such as the MabSelect SuReTM ligand sold by GE Healthcare. If you are using a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuReTM you should use the Catalog #F400 kit. If you are evaluating our Protein A assays for the first time, we recommend using this kit, since it offers other robustness and procedural advantages over the older kits. The LOD is ~50pg/mLWe have improved the sample treatment procedure which reduces tedious pipetting while increasing throughput of the sample processing step. Validation of the new method shows excellent precision with improved overall robustness of the assay. This new method has demonstrated superior spike and recovery in problematic sample matrices and also overcomes 'front-to-back' drift reported by some labs. Labs equipped with microplate adaptable centrifuges can now process up to 96 tests simultaneously. Instead of using individual microfuge tubes, the new method uses a 96 well "PCR" Sample Treatment Plate and an adhesive seal, included in the kit. This procedure also minimizes problems of inadvertent disruption of the pelletized denatured proteins when handling microfuge tubes.